Related Experiment Video
Updated: May 6, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
Plant regeneration fromArabidopsis thaliana protoplasts
1Department of Biology, College of Charleston, 29424, Charleston, South Carolina, USA.
Abstract:
Arabidopsis thaliana protoplasts, isolated from a cell suspension, regenerated cell walls and then exhibited sustained cell divisions. Within 10 days after isolation as many as 40% of the protoplast-derived cells divided. Protoplast-derived calli exhibited a wide range of developmental responses, including apparent embryogenesis. The cell suspension has been cryopreserved.
More Related Videos
07:42Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Published on: September 17, 2016
08:52Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae
Published on: December 22, 2023