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Production of canthin-6-one alkaloids by cell suspension cultures of Brucea javanica (L.) Merr
K C Liu1, S L Yang, M F Roberts
1Department of Pharmacognosy, The School of Pharmacy, University of London, 29-39 Brunswick Square, WC1N 1AX, London, UK.
Abstract:
Cell suspension cultures of Brucea javanica (L.) Merr. were used to determine culture growth characteristics and production of canthin-6-one alkaloids. The major alkaloids produced were canthin-6-one, 11-hydroxycanthin-6-one, 5-methoxycanthin-6-one and 11-methoxycanthin-6-one. Alkaloids were synthesised throughout the 36 day growth cycle of the cells with maximum amounts within the cells occurring between days 20 to 28; approximately 10% of the alkaloids were in the medium at day 24 rising to 45% at day 32.
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