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Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
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Time-resolved tryptophan fluorescence in flavodoxins
R Leenders1, J Roslund, A J Visser
1Department of Biochemistry, Agricultural University, Dreijenlaan 3, 6703, HA Wageningen.
Abstract:
The time-resolved fluorescence characteristics of tryptophan in flavodoxins isolated from the bacteriaDesulfovibrio gigas, Desulfovibrio vulgaris, Clostridium beijerinckii, andMegasphaera elsdenii were examined. The fluorescence decays were recorded using pulsed synchrotron radiation as the excitation source and time-correlated single-photon counting in detection. The results were analyzed as lifetime distributions using the maximum entropy method. Comparison of the fluorescence decays of normal and flavin mononucleotide-depleted flavodoxins demonstrates that radiationless energy transfer from tryptophan to flavin occurs in all flavodoxins investigated. On comparing the lifetime distribution patterns of apo and holoflavodoxins, it was noticed that a certain amount of apoprotein is present in all holoflavodoxin samples. The three-dimensional structure of two flavodoxins allowed us to compare experimental with theoretical transfer rates and the results were in fair agreement.
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