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L-Dityrosine: A time-resolved fluorescence investigation.

A J Kungl1, G Landl, A J Visser

  • 1Institut für Physikalische Chemie der Universität Wien, Währingerstraße 42, A-1090, Wien, Austria.

Journal of Fluorescence
|November 19, 2013
PubMed
Summary
This summary is machine-generated.

We studied the fluorescence of LL-dityrosine, revealing a dual fluorescence behavior suggesting an excited-state reaction. This reaction, likely a torsional motion, involves a precursor-successor pair in LL-dityrosine.

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Area of Science:

  • Photophysics
  • Biomolecular Spectroscopy

Background:

  • Dityrosine exhibits complex fluorescence properties.
  • Understanding its excited-state dynamics is crucial for biochemical applications.

Purpose of the Study:

  • Investigate the time-resolved fluorescence of LL-dityrosine in aqueous solution.
  • Elucidate the photophysical mechanisms underlying its dual fluorescence behavior.

Main Methods:

  • Time-resolved fluorescence spectroscopy
  • Fluorescence anisotropy decay analysis
  • Exponential Series Method with Tikhonov regularization

Main Results:

  • Three exponential decay components were required to fit the fluorescence data.
  • A subnanosecond rise phase was observed for red-edge fluorescence.
  • Dual fluorescence indicates an intramolecular precursor-successor pair, likely due to torsional motion.
  • Analysis revealed two rotational correlation times, one with a negative preexponential term.
  • Lifetime distributions showed three distinct groups, including a formally negative short-lived ensemble.
  • A photophysical model explained biexponential decay via overlapping precursor and successor emission.

Conclusions:

  • LL-dityrosine exhibits dual fluorescence attributed to a consecutive intramolecular excited-state reaction.
  • Torsional motion along the biphenolic bond is proposed as the reaction mechanism.
  • The findings provide a comprehensive photophysical model for dityrosine fluorescence.