Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Energy transfer leaves fingerprints in cyanine photoswitching behavior.

PLoS computational biology·2026
Same author

Multicolor Super-resolution Fluorescence Imaging of Cells by Expansion Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

DNAM-1 mediates NK-cell activation and host-pathogen interaction via direct binding to fungal cell wall proteases.

Communications biology·2026
Same author

Traction Force Microscopy with DNA FluoroCubes.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Loss of GPRC5D enhances the proliferative capacity and competitive fitness of myeloma upon anti-GPRC5D immunotherapy.

Leukemia·2026
Same author

Adventitious carbon breaks symmetry in oxide contact electrification.

Nature·2026
Same journal

Spectroscopic Investigation of the In Vivo Light-Dependent Photodynamics of the Marine Diatom Phaeodactylum tricornutum.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Atomistic Insights into the Thermal Decomposition and Runaway Mechanism of Peroxypropionic Acid.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Hydrazine Adsorption on Hexagonal Ice (0001): First-Principles Investigations on Stability, Dynamics, and Chirality Changes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Sustainable Ball Milling-Assisted Synthesis of Bread Waste-Derived Highly Porous Carbons for Adsorption-Based Applications.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

RNALig: An ML-Driven Structure-Based Scoring Function for Estimating Binding Affinities of RNA-Ligand Complexes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Photoswitchable Polar Azobenzene-Based Liquid Crystals for Electro-Optic and Optical Data Storage Applications.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
See all related articles

Related Experiment Video

Updated: May 6, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.1K

A blueprint for cost-efficient localization microscopy.

Thorge Holm1, Teresa Klein, Anna Löschberger

  • 1Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|November 15, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a compact, affordable localization microscopy system. This super-resolution imaging tool can visualize cellular structures and train students in advanced microscopy techniques.

Keywords:
cost-efficiencyfluorescencelaser spectroscopylocalization microscopysuper-resolution imaging

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.7K
Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.4K

Related Experiment Videos

Last Updated: May 6, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.1K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.7K
Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.4K

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Cell Biology

Background:

  • Super-resolution microscopy enables visualization of cellular structures beyond the diffraction limit of light.
  • Traditional super-resolution setups can be complex and expensive, limiting accessibility.

Purpose of the Study:

  • To introduce a miniaturized and cost-effective localization microscopy setup.
  • To demonstrate its capability for subdiffraction resolution fluorescence imaging of cellular nanostructures.

Main Methods:

  • Development of a compact localization microscopy system using affordable components.
  • Application of the setup for fluorescence imaging of biological samples.

Main Results:

  • Successful subdiffraction resolution imaging of various cellular nanostructures was achieved.
  • The miniaturized setup proved feasible for high-resolution fluorescence microscopy.

Conclusions:

  • The developed localization microscopy system offers an accessible platform for advanced cellular imaging.
  • This cost-effective setup is suitable for practical training in super-resolution fluorescence microscopy.