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Spectroscopic studies on the interaction of Ga3+-hypocrellin A with myoglobin
Wenli Xie1, Shaohua Wei1, Jihua Liu2
1College of Chemistry and Materials Science, Analysis and Testing Center, Jiangsu Key Laboratory Biofunctional Materials, Key Laboratory of Applied Photochemistry, JiangSu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University, Wenyuan Road No. 1, Nanjing 210023, PR China.
Abstract:
In this article, the interaction mechanism of Ga(3+)-hypocrellin A (Ga(3+)-HA) with myoglobin (Mb) is studied in detail through various spectroscopic technologies. UV-vis absorption and fluorescence spectra demonstrate the interaction process. The Stern-Volmer plot and the time-resolved fluorescence study suggest the fluorescence quenching mechanism of Mb by Ga(3+)-HA is a static quenching procedure, and the electronic transfer forces play a major role in binding Ga(3+)-HA to Mb. Furthermore, synchronous fluorescence studies and circular dichroism (CD) spectra reveal that the conformation of Mb is changed after its conjugation with Ga(3+)-HA.
