Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.)
1Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan.
Abstract:
We obtained cybrid plants by electrofusing γ-irradiated protoplasts of a cytoplasmic male-sterile line "A-58 CMS" (Oryza sativa L.) and iodoacetamide (IOA)-treated protoplasts of the fertile (normal) rice cultivar "Fujiminori". The cybridity of the plants was confirmed by mitochondrial (mt) DNA restriction endonuclease, and plasmid-like DNA analyses, and by isozyme, cytological and morphological investigations. The chromosome number of the cybrid plants is 24.
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