Characterization of immature embryos of interior spruce by SDS-PAGE and microscopy in relation to their competence
D R Roberts1, B S Flinn, D T Webb
1Forest Biotechnology Centre, British Columbia Research Corporation, 3650 Wesbrook Mall, V6S 2L2, Vancouver, B.C., Canada.
Abstract:
SDS-PAGE analysis of total proteins from cotyledonary embryo explants reveals that their competence to form somatic embryos is limited to a specific stage of development prior to the accumulation of storage proteins. When protein profiles of embryo explants of different open pollinated families from the same collection date are compared, there is a close relationship between the absence of storage proteins and their ability to produce embryogenic callus. In addition, the appearance of storage proteins in embryos from subsequent collections is associated with their loss of competence. Light microscopy combined with staining for total protein demonstrates that competent immature embryos have cotyledons but do not contain protein bodies.
