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Related Experiment Video

Updated: May 6, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Fiber-optic fluorescing sensors for nitrate and nitrite detection.

B Mahieuxe¹, M C Carré, M L Viriot

¹GRAPP-DCPR-URA 328, ENSIC-INPL, 1 rue Grandville, F-54000, Nancy, France.

Journal of Fluorescence

|November 16, 2013

Summary

This summary is machine-generated.

We developed novel fiber-optic chemical sensors for detecting nitrate and nitrite anions using fluorescence. These sensors offer sensitive, remote analysis capabilities for environmental and biological applications.

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

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Published on: May 5, 2016

Area of Science:

Analytical Chemistry
Materials Science
Biotechnology

Background:

Fiber-optic sensors enable remote chemical analysis with broad applications in chemistry and biology.
Fluorometric methods are suitable for detecting substances at parts per million/billion levels.
Nitrate and nitrite analysis is crucial in various scientific fields.

Purpose of the Study:

To report initial results in developing fiber-optic chemical sensors.
To analyze nitrate and nitrite anions using fluorescence modifications.
To establish simple instrumentation for fiber-optic sensing.

Main Methods:

Utilized fluorometric detection for analyzing nitrate and nitrite anions.
Developed extrinsic active optical fibers via silanization (APTES) and chemical dye linkage.
Designed a dedicated optical device for fiber-optic measurements.

Main Results:

Demonstrated nitrate detection via fluorescein fluorescence inhibition.
Showcased nitrite detection through enhanced fluorescence with 2,3-diaminonaphthalene.
Presented data on detection thresholds, coating efficiency, and sensor stability.

Conclusions:

Successfully developed fiber-optic sensors for nitrate and nitrite detection.
The developed sensors show promise for sensitive and remote chemical analyses.
Further characterization of detection limits, efficiency, and stability is ongoing.