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Updated: May 6, 2026
A Fluorescence-based Assay of Phospholipid Scramblase Activity
Published on: September 20, 2016
Diphenylhexatriene-phosphatidylcholine fluorescence in POPC vesicles: Comparison of the exponential-series and the
1Institute of Physical Chemistry, University Wien, Währingerstraße 42, A-1090, Wien, Austria.
Abstract:
We have investigated the time-resolved fluorescence of diphenylhexatriene (DPH) covalently linked to phosphatidylcholine (PC) in palmitoyloleoylglycerophosphocholine (POPC) vesicles with special consideration of the comparison of two methods for distributional lifetime analysis: the exponential-series method (ESM) and the maximum-entropy method (MEM). Generally, both methods were found to reveal equivalent results on high-quality data. Different are the shapes of the recovered distributions (symmetry and width) as well as the time effort for the numerical analysis.
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