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Rapid determination of anti-enzyme titer in serum using wholeStaphylococcus aureus cells

¹Department of Biochemistry, University of North Carolina, School of Medicine, 27514, Chapel Hill, NC.

Applied Biochemistry and Biotechnology

|November 16, 2013

Abstract:

Heat-killed, formalin-fixedStaphyloccous aureus (Cowan) cells are shown to be an excellent immunoadsorbent for quickly and accurately measuring the titer of antibodies directed against enzymes. The simple procedure is illustrated by following the course of immunization of a rabbit against mouse uridine 5't -monophosphate synthase. The antibody titer of a serum sample can be determined in roughly 1 h plus the time to perform the assays of enzyme (antigen) activity.

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