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Engineering the MEP pathway enhanced ajmalicine biosynthesis.

Kai Chang1, Fei Qiu, Min Chen

  • 1Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Engineering and Technology Research Centre for Sweetpotato, School of Life Sciences, Southwest University, Chongqing, People's Republic of China; Chengdu Grain Storage Research Institute, State Administration of Grain Reserves, Chengdu, People's Republic of China.

Biotechnology and Applied Biochemistry
|November 19, 2013
PubMed
Summary
This summary is machine-generated.

Genetic modification of the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway in Catharanthus roseus hairy roots enhanced ajmalicine production. Co-overexpression of MEP pathway genes (DXR/MECS) with STR significantly boosted ajmalicine yield.

Keywords:
Catharanthus roseusDXRMECSSTRalkaloidspathway engineering

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Area of Science:

  • Plant Biotechnology
  • Metabolic Engineering
  • Natural Product Biosynthesis

Background:

  • Ajmalicine is a valuable terpenoid indole alkaloid with significant pharmaceutical applications.
  • The 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway is crucial for isoprenoid precursor supply.
  • Genetic engineering offers a promising avenue for enhancing alkaloid production in medicinal plants.

Purpose of the Study:

  • To investigate the impact of overexpressing MEP pathway genes (DXR, MECS) and STR on ajmalicine biosynthesis in *Catharanthus roseus* hairy root cultures.
  • To determine the synergistic effects of co-overexpressing MEP pathway genes and STR on ajmalicine accumulation.
  • To optimize metabolic flux towards ajmalicine production through targeted gene manipulation.

Main Methods:

  • Genetic modification of *Catharanthus roseus* hairy root cultures using *Agrobacterium rhizogenes*.
  • Overexpression of DXR and MECS genes from *Taxus* species and STR gene from *Catharanthus roseus*.
  • Quantitative analysis of ajmalicine accumulation in transgenic hairy root cultures.

Main Results:

  • Single overexpression of STR led to a twofold increase in ajmalicine accumulation.
  • Overexpression of individual MEP pathway genes (DXR or MECS) also significantly enhanced ajmalicine biosynthesis.
  • Co-overexpression of DXR/MECS with STR resulted in higher ajmalicine levels compared to single-gene overexpression, indicating enhanced metabolic flux.

Conclusions:

  • Engineering the MEP pathway by overexpressing DXR or MECS promotes metabolic flux into ajmalicine biosynthesis.
  • Co-overexpression of MEP pathway genes (DXR/MECS) and STR is a more efficient strategy for enhancing ajmalicine yield in *Catharanthus roseus* hairy roots.
  • This study provides a valuable strategy for improving the production of terpenoid indole alkaloids through metabolic engineering.