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Updated: May 5, 2026
Inducing Apical Periodontitis in Mice
Published on: August 6, 2019
Histologic characterization of engineered tissues in the canal space of closed-apex teeth with apical periodontitis
João Eduardo Gomes-Filho1, Paulo Carvalho Tobias Duarte, Edilson Ervolino
1Department of Endodontics, Araçatuba School of Dentistry, Universidade Estadual Paulista, Araçatuba, São Paulo, Brazil.
Introduction:
The aim of this study was to investigate the capacity of endodontic regenerative procedures combining an induced blood clot, platelet-rich plasma (PRP), and bone marrow aspirate (BMA) to regenerate dental pulp in canine closed-apex necrotic teeth.
Methods:
Apical periodontitis was induced in 20 upper and lower premolars of 2 dogs. After biomechanical preparation, enlargement to a #60 file, and disinfection with a triantibiotic paste for 28 days, the roots were randomly assigned to 4 treatment groups: blood clot (BC), BC + PRP gel, BC + BMA gel, and BC + BMA/PRP gel. Negative controls were also included. After a 3-month follow-up period, the animals were killed.
Results:
Histologic analysis showed the presence of newly formed vital tissues (connective, cement-like, and bone-like tissue) in 23 of the 32 treated roots (71.87%). There was no statistically significant difference between the treatment groups.
Conclusions:
New vital tissues were formed and characterized as connective, cementum-like, or bone-like, but not as pulp-like tissue; PRP and/or BMA did not improve the tissue ingrowth.
