Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

4.0K
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
4.0K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Theoretical Calculation and Simulation of Peak Distortion of Absorption Spectra of Complex Mixtures.

Applied spectroscopy·2024
Same author

Exploring tissue permeability of brain tumours in different grades: Insights from pore-scale fluid dynamics analysis.

Acta biomaterialia·2024
Same author

Simultaneous Application of Raman and Laser-Induced Breakdown Spectroscopy in the Gas Phase with a Single Laser and Detector.

Applied spectroscopy·2024
Same author

Multimodal image and spectral feature learning for efficient analysis of water-suspended particles.

Optics express·2023
Same author

Unsupervised feature learning and clustering of particles imaged in raw holograms using an autoencoder.

Journal of the Optical Society of America. A, Optics, image science, and vision·2021
Same author

Laser applications to chemical, security, and environmental analysis: introduction to the feature issue.

Applied optics·2021

Related Experiment Video

Updated: May 5, 2026

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

14.0K

Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects.

Florian M Zehentbauer1, Claudia Moretto1, Ryan Stephen1

  • 1School of Engineering, University of Aberdeen, Fraser Noble Building, Aberdeen AB24 3UE, Scotland, United Kingdom.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|November 19, 2013
PubMed
Summary

Rhodamine 6G (R6G) fluorescence is sensitive to solvents and concentration. Methanol enhances intensity, while DMSO shifts the peak wavelength, and aggregation at high concentrations causes significant spectral changes.

Keywords:
AlcoholDye laserFlow visualizationLaser-induced fluorescenceRhodamine 590Rhodamine 6G

More Related Videos

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

10.5K
A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements
06:06

A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements

Published on: July 19, 2016

8.8K

Related Experiment Videos

Last Updated: May 5, 2026

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

14.0K
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

10.5K
A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements
06:06

A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements

Published on: July 19, 2016

8.8K

Area of Science:

  • Photochemistry
  • Spectroscopy
  • Materials Science

Background:

  • Rhodamine 6G (R6G) is a widely used fluorescent dye in dye lasers and as a tracer in environmental hydraulics.
  • Understanding its spectroscopic properties is crucial for optimizing laser efficiency and measurement accuracy.

Purpose of the Study:

  • To investigate the effects of various organic solvents and R6G concentrations on its fluorescence characteristics.
  • To determine how solvent polarity and R6G aggregation influence emission spectra.

Main Methods:

  • Studied R6G in eight organic solvents (alcohols, acetone, DMSO) at a constant concentration.
  • Examined R6G in aqueous solutions across a wide concentration range, from dilute to near saturation.

Main Results:

  • Solvent polarity caused minor spectral shifts, with methanol yielding the highest fluorescence intensity and DMSO the lowest.
  • Peak emission wavelength varied from 568 nm (methanol) to 579 nm (DMSO).
  • In aqueous solutions, R6G concentration tuned emission from ~550 nm (dilute) to ~620 nm (high concentration) due to aggregate formation.

Conclusions:

  • Solvent choice and R6G concentration significantly impact fluorescence properties.
  • Rhodamine 6G's spectral tunability is demonstrated, with aggregation playing a key role at higher concentrations.