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Updated: May 5, 2026
Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Published on: August 13, 2010
Flexible ordering of antibody class switch and V(D)J joining during B-cell ontogeny
Satyendra Kumar1, Robert Wuerffel, Ikbel Achour
1Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, Illinois 60612, USA;
Abstract:
V(D)J joining is mediated by RAG recombinase during early B-lymphocyte development in the bone marrow (BM). Activation-induced deaminase initiates isotype switching in mature B cells of secondary lymphoid structures. Previous studies questioned the strict ontological partitioning of these processes. We show that pro-B cells undergo robust switching to a subset of immunoglobulin H (IgH) isotypes. Chromatin studies reveal that in pro-B cells, the spatial organization of the Igh locus may restrict switching to this subset of isotypes. We demonstrate that in the BM, V(D)J joining and switching are interchangeably inducible, providing an explanation for the hyper-IgE phenotype of Omenn syndrome.
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