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Updated: May 5, 2026
Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
Published on: October 20, 2019
Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids
J Fahleson1, J Dixelius, E Sundberg
1Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 7003, S-750 07, Uppsala, Sweden.
Abstract:
Fourty-one somatic hybrids from two species combinations, Brassica oleracea + B. campestris and B. napus + Eruca sativa, were analysed for chromosome number and nuclear DNA content. The DNA content was measured in a flow cytometer using two internal standards as references and when related to the chromosome number a correlation of 0.91 was found. The chromosome number of the hybrids could be determined with an accuracy of ±10% by using flow cytometry, and the smallest statistically significant difference in DNA content between two individuals was 0.23 pg DNA/cell.
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