Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

New quality measure for SNP array based CNV detection.

Bioinformatics (Oxford, England)·2016

Same author

Chromosomal contacts connect loci associated with autism, BMI and head circumference phenotypes.

Molecular psychiatry·2016

Same author

The fetal thymus has a unique genomic copy number profile resulting from physiological T cell receptor gene rearrangement.

Scientific reports·2016

Same author

16p11.2 Locus modulates response to satiety before the onset of obesity.

International journal of obesity (2005)·2015

Same author

Individual differences in impulsive action and dopamine transporter function in rat orbitofrontal cortex.

Neuroscience·2015

Same author

Familial aggregation of stroke amongst young patients in Lund Stroke Register.

European journal of neurology·2015

Same journal

Combined genome and transcriptome analysis of boll weight and lint percentage traits in Gossypium barbadense.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

Same journal

The allelic variation of anthocyanidin reductase underlies anthocyanin biosynthesis and purple leaf trait in Brassica napus.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

Same journal

Unveiling core genomic regions shaping plant architecture, productivity, and seed quality traits in sesame (Sesamum indicum L.): insights from Meta-QTL study into breeding targets.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

Same journal

Watkins wheat landraces: a treasure of stripe rust resistance alleles identified using multi-model association analyses.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

Same journal

Selection of four mutant alleles of fatty acid desaturase genes for a stable high oleic and low linolenic acid soybean seed oil trait.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

Same journal

Harnessing artificial intelligence in plant breeding: innovations in digital phenotyping and breeding methodologies.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 5, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

Cloning quantitative trait loci by insertional mutagenesis.

M Soller¹, J S Beckmann

¹Department of Genetics, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 19, 2013

Summary

This summary is machine-generated.

This study proposes insertional mutagenesis to clone quantitative trait loci (QTL). It requires generating and screening numerous DNA inserts, with multi-stage testing to identify true genetic effects on traits.

More Related Videos

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

Related Experiment Videos

Last Updated: May 5, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Published on: February 3, 2023

Area of Science:

Genetics and Genomics
Molecular Biology
Quantitative Genetics

Background:

Cloning quantitative trait loci (QTL) is crucial for understanding complex traits.
Insertional mutagenesis offers a potential, albeit challenging, approach to QTL discovery.
The direct search for mutagenic effects requires robust statistical methods to overcome small effect sizes.

Purpose of the Study:

To explore the theoretical feasibility of using insertional mutagenesis for cloning QTL.
To outline the necessary experimental design and statistical considerations for such an approach.
To identify key factors influencing the efficiency and power of insertional mutagenesis in QTL studies.

Main Methods:

Theoretical modeling of insertional mutagenesis for QTL cloning.
Statistical framework involving multi-stage replicate testing to control Type I error and maintain power.
Consideration of experimental parameters such as insert numbers, replicate sizes, and trait scoring.

Main Results:

Approximately 10,000 inserts may require screening under specific statistical thresholds (n ≧ 30, α=0.01) for initial detection.
Efficiency increases with the number of traits scored and multiple inserts per parent.
Inbred lines and selfing are recommended for experimental replication and error reduction.

Conclusions:

Insertional mutagenesis is a theoretically viable, though resource-intensive, strategy for QTL cloning.
Successful implementation hinges on generating numerous inserts and employing rigorous statistical validation.
Arabidopsis thaliana is highlighted as a suitable model organism, with somaclonal variation noted as a potential challenge.