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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
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An electrospray ion source for magnetic sector mass spectrometers
1Central Research and Development Deparment, E. I. du Pont de Nemours & Company, Inc., P. O. Box 80228, 19880-0288, Wilmington, DE.
Journal of the American Society for Mass Spectrometry
|November 19, 2013
Summary
High-performance magnetic sector mass spectrometers coupled with atmospheric pressure electrospray ionization enable detailed analysis of proteins like carbonic anhydrase and ovalbumin. This setup achieves high resolution for accurate mass measurements of peptides and proteins.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Mass Spectrometry
Background:
- Electrospray ionization (ESI) is a crucial technique for analyzing biomolecules.
- Magnetic sector mass spectrometers offer high resolution but interfacing them with ESI can be challenging.
Purpose of the Study:
- To demonstrate the effective interfacing of a high-performance magnetic sector mass spectrometer with an atmospheric pressure electrospray ionization source.
- To analyze proteins and peptides using this integrated system.
Main Methods:
- Utilized a double focusing magnetic sector mass spectrometer.
- Employed a single stage of mechanical pumping for the interface between atmospheric pressure and high vacuum.
- Obtained electro spray mass spectra for carbonic anhydrase and ovalbumin.
- Recorded full scan spectra for lysozyme.
Main Results:
- Successfully obtained mass spectra for proteins carbonic anhydrase (MW ~29,000) and ovalbumin (MW ~45,000).
- Achieved high resolution (exceeding 10,000 at 10% valley) for accurate mass measurements of peptides and proteins.
- Demonstrated the capability to analyze as little as 15 femtomoles of lysozyme.
Conclusions:
- High-performance magnetic sector mass spectrometers are well-suited for interfacing with atmospheric pressure electrospray ionization sources.
- This integrated system provides high resolution and sensitivity for protein and peptide analysis.
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