Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Direct analysis of volatile organic compounds in foods by headspace extraction atmospheric pressure chemical ionisation mass spectrometry.

Rapid communications in mass spectrometry : RCM·2017

Same author

Antinuclear antibodies associate with loss of response to antitumour necrosis factor-alpha therapy in psoriasis: a retrospective, observational study.

The British journal of dermatology·2009

Same author

Percent absent end-diastolic velocity in the umbilical artery waveform as a predictor of intrauterine fetal demise of the donor twin after selective laser photocoagulation of communicating vessels in twin-twin transfusion syndrome.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2007

Same author

Azapirones for generalized anxiety disorder.

The Cochrane database of systematic reviews·2006

Same author

Apolipoprotein E gene polymorphisms are associated with psoriasis but do not determine disease response to acitretin.

The British journal of dermatology·2006

Same author

Corneodesmosin (CDSN) gene association with psoriasis vulgaris in Caucasian but not in Japanese populations.

Clinical and experimental dermatology·2005

Search research articles

Related Experiment Video

Updated: May 5, 2026

Glass-Based Devices to Generate Drops and Emulsions

Glass-Based Devices to Generate Drops and Emulsions

Published on: April 5, 2022

Design and performance of a novel electrospray interface.

M H Allen¹, M L Vestal

¹Vestec Corporation, 9299 Kirby Drive, 77054, Houston, TX.

Journal of the American Society for Mass Spectrometry

|November 19, 2013

Summary

A novel electrospray system uses heat for desolvation without heated gas. This new design enhances signal intensity and allows for protein analysis and fragmentation data acquisition.

Area of Science:

Analytical Chemistry
Mass Spectrometry
Separation Science

Background:

Traditional electrospray ionization (ESI) often relies on heated gas for desolvation.
Existing ESI systems can be complex and require specific gas flows.
Optimizing ESI performance is crucial for sensitive molecular analysis.

Purpose of the Study:

To introduce a new electrospray system utilizing heat for desolvation.
To detail the operational principles and performance characteristics of the novel system.
To compare the new system with existing electrospray technologies.

Main Methods:

Development of a novel electrospray system employing heat for desolvation.
Investigation of operational parameters including ion source and spray chamber temperatures.

More Related Videos

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

Published on: June 16, 2014

Related Experiment Videos

Last Updated: May 5, 2026

Glass-Based Devices to Generate Drops and Emulsions

Glass-Based Devices to Generate Drops and Emulsions

Published on: April 5, 2022

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

Published on: June 16, 2014

Analysis of mobile phase composition and flow rate effects on signal intensity.

Electrospraying of proteins from aqueous solutions, including a bacterial protease.

Assessment of fragmentation data acquisition via collisional induced dissociation (CID) within the interface.

Main Results:

The new electrospray system effectively uses heat for desolvation without requiring counterflow gas.
Signal intensity demonstrates clear dependence on ion source temperature, spray chamber temperature, mobile phase composition, and flow rate.
Successful electrospraying of proteins from aqueous solutions was achieved, including a protein with a rigid tertiary structure.
The system's capability for obtaining fragmentation data through interface-based CID was demonstrated.

Conclusions:

The developed electrospray system offers an alternative desolvation method using heat, simplifying operation.
The system's performance is tunable via temperature and mobile phase parameters, enabling optimized ion generation.
The ability to analyze complex proteins and acquire fragmentation data highlights its versatility in mass spectrometry applications.