Related Experiment Video
Updated: May 5, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
Published on: June 19, 2010
Are the electrospray mass spectra of proteins related to their aqueous solution chemistry?
R Guevremont1, K W Siu, J C Le Blanc
1Institute for Environmental Chemistry, National Research Council of Canada, Montreal Road, KIA OR9, Ottawa, Ontario, Canada.
Abstract:
Institute for Environmental Chemistry, National Research Council of Canada, Ottawa, Ontario, Canada The shape of the profile described by the relative abundances of multiply charged ions of proteins in the electrospray mass spectrum can be described by means of one or more Gaussian functions. An aqueous solution equilibrium model of the distribution of multiply charged ions of equine cytochrome c and myoglobin has been shown to match qualitatively the shape of the distribution of these ions in an electrospray mass spectrum. Monotonic functions such as the quadrupole mass spectrometric transmission efficiency may alter the centroid of the profile, but the shape of the ion abundance pattern appears to be controlled by the aqueous solution chemistry of the proteins. NRCC No. 32938.
More Related Videos
09:18Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Published on: April 17, 2017
07:33Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
Published on: October 15, 2018
Related Concept Videos
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometry: Overview
Mass Spectrometry: Isotope Effect
Mass Spectrum: Interpretation
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...