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Diffusion-influenced fluorescence quenching dynamics in one to three dimensions.

B Medhage¹, M Almgren

¹Department of Physical Chemistry, Uppsala University, Box 532, S-751 21, Uppsala, Sweden.

Journal of Fluorescence

|November 19, 2013

Summary

This study presents a new approximation for two-dimensional (2D) fluorescence quenching, improving analysis of diffusion-influenced quenching. The findings enhance the ability to accurately model and differentiate between various spatial dimensions in quenching studies.

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Area of Science:

Physical Chemistry
Chemical Kinetics
Spectroscopy

Background:

Diffusion-influenced quenching is crucial in chemical kinetics.
Analyzing two-dimensional (2D) quenching is challenging due to complex fitting functions.
Previous models were limited to the Smoluchowski boundary condition.

Purpose of the Study:

To present an approximation for 2D fluorescence quenching under the Collins-Kimball boundary condition.
To investigate the impact of model choice on fitting results.
To explore methods for discriminating between different spatial dimensions in quenching.

Main Methods:

Development of an approximation for 2D quenching with the Collins-Kimball boundary condition.
Utilizing nonlinear least-squares analysis for simulated fluorescence decay data.
Investigating inherent properties of fitting functions.

Main Results:

The proposed approximation allows for analysis of 2D quenching beyond the diffusion-controlled limit.
Simulated data analysis reveals consequences of using incorrect models.
The study demonstrates the potential to distinguish between different dimensionalities.

Conclusions:

The developed approximation offers a more versatile approach to studying 2D diffusion-influenced quenching.
Accurate model selection is critical for reliable interpretation of fluorescence decay data.
This work provides tools for better understanding spatial effects in quenching phenomena.