Phage as a template to grow bone mineral nanocrystals
Binrui Cao1, Hong Xu, Chuanbin Mao
1Department of Chemistry & Biochemistry, Stephenson Life Sciences Center, University of Oklahoma, Norman, OK, USA.
Abstract:
Phage display is a biotechnique that fuses functional peptides on the outer surface of filamentous phage by inserting DNA encoding the peptides into the genes of its coat proteins. The resultant peptide-displayed phage particles have been widely used as biotemplates for the synthesis of functional hybrid nanomaterials. Here, we describe the bioengineering of M13 filamentous phage to surface-display bone mineral (hydroxyapatite (HAP))-nucleating peptides derived from dentin matrix protein-1 and using the engineered phage as a biotemplate to grow HAP nanocrystals.
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