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Updated: May 5, 2026

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Published on: June 24, 2012

Norovirus gene expression and replication.

Lucy G Thorne¹, Ian G Goodfellow¹

¹Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, UK.

The Journal of General Virology

|November 19, 2013

Summary

This summary is machine-generated.

Noroviruses cause widespread gastroenteritis, but their life cycle remained unclear due to culturing difficulties. Recent advances in norovirus research have significantly improved understanding of viral gene expression and replication.

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Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

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Published on: November 16, 2017

Area of Science:

Virology
Molecular Biology
Infectious Diseases

Background:

Noroviruses are a leading cause of acute gastroenteritis globally.
The inability to culture human noroviruses (HuNVs) has historically limited understanding of their life cycle.
Significant technological advancements have recently improved norovirus research.

Purpose of the Study:

To review the current knowledge of the intracellular life cycle of noroviruses.
To emphasize the mechanisms of viral gene expression and genome replication.
To highlight recent progress in understanding norovirus biology.

Main Methods:

Utilizing a human norovirus (HuNV) replicon system.
Employing improved biochemical and cell-based assays.
Leveraging the discovery of a murine norovirus model for cell culture replication.

Main Results:

Enhanced understanding of norovirus genome translation and replication mechanisms.
Improved insights into norovirus interactions with host cell processes.
Progress in studying viral gene expression pathways.

Conclusions:

Technological advances have overcome previous limitations in norovirus research.
The molecular mechanisms of norovirus replication and gene expression are becoming clearer.
Further research promises deeper insights into norovirus pathogenesis and control.