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Systematic reference sample generation for multiplexed serological assays.

Hannes Planatscher1, Stefanie Rimmele, Gerd Michel

  • 1NMI Natural and Medical Sciences Institute at the University of Tuebingen, Markwiesenstr. 55, 72770 Reutlingen, Germany.

Scientific Reports
|November 20, 2013
PubMed
Summary
This summary is machine-generated.

Developing reliable quality controls for multi-antigen serological assays is crucial. This study presents a mathematical method to optimize sample pooling for creating accurate quality controls, demonstrated for a 71-plex tuberculosis antigen array.

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Area of Science:

  • Immunology
  • Biotechnology
  • Computational Biology

Background:

  • * Serological assays require quality controls with specific human antibodies.
  • * Array-based assays targeting numerous antigens present pooling challenges.
  • * Current methods rely on empirical identification of optimal serum pools.

Purpose of the Study:

  • * To develop a systematic mathematical approach for calculating optimal sample pool composition.
  • * To create accurate quality controls for multiplex antigen bead arrays.
  • * To address the challenge of pooling diverse patient sera for quality control.

Main Methods:

  • * A novel mathematical model was developed for sample pool composition calculation.
  • * The approach was applied to a 71-plex Tuberculosis antigen bead array.
  • * Limited numbers of positive human sera were utilized.

Main Results:

  • * A systematic method for calculating optimal sample pool composition was successfully established.
  • * The approach enabled the accurate compilation of quality controls.
  • * Suitable quality controls were created for a complex 71-plex Tuberculosis antigen assay.

Conclusions:

  • * The mathematical approach provides a systematic and accurate solution for quality control pool composition.
  • * This method optimizes the use of limited patient sera for multiplex assay validation.
  • * The findings are applicable to the development of quality controls for various array-based serological assays.