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In vitro regeneration of plants from hypocotyl segments of Amaranthus paniculatus
S Bagga1, K Venkateswarlu, S K Sopory
1Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India.
Abstract:
Hypocotyl segments, 5 to 8 mm length from 4 to 7 day old seedlings, callused on B5 medium supplemented with Kn (0.5 ppm) and NAA (0.1 ppm). Even without transfer, shoots were formed in such cultures. About 20% of the cultures produced multiple shoots. In medium with 1 ppm each of Kn and NAA direct shoots were formed at one end of the hypocotyl segment and callusing was initiated at the other end. The plants obtained in either medium formed roots and could be transferred to soil for further growth.
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