Related Experiment Video
Updated: May 5, 2026

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Published on: February 24, 2021
Changes in adenine-nucleotide content in the apical bud of Sinapis alba L. during floral transition
1Université de Liége, Dèpartement de Botanique, Laboratoire de Physiologie végétale et Centre de Physiologie Végétale Appliquée (IRSIA), Sart Tilman, Bât. B22, B-4000, Liège, Belgium.
Abstract:
The total adenylate pool of the apical buds of vegetative plants of Sinapis alba L. continuously grown in short days fluctuates over a 24-h cycle with the minimum occurring at the end of the dark period. In the buds of plants induced to flower by a single long-day treatment, total adenylate pool increases above the control level 16 h after the start of the long day, resulting mainly from a rise in ATP and ADP contents. This occurs 6 h after the increase in the soluble carbohydrate content previously shown to occur in the apical buds of plants induced to flower (Bodson 1977, Planta 135, 19-23). A transient rise of the energy charge occurs 22 h after the start of the inductive long day.

