Related Experiment Video

Updated: May 5, 2026

Dissection of Hippocampal Dentate Gyrus from Adult Mouse
07:42

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

84.6K

Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization

Hiroyuki Yoshida1, Aya Nagaoka, Sachiko Nakamura

  • 1Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., 3-28, 5-chome, Kotobuki-cho, Odawara-shi, Kanagawa 250-0002, Japan.

FEBS Open Bio
|November 20, 2013
PubMed

Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.

Keywords:
CHC, clathrin heavy chainCPC, cetylpyridinium chlorideCS, chondroitin sulfateCatabolismDS, dermatan sulfateExtracellular matrixFA, fluoresceinamineFBS, fetal bovine serumGAG, glycosaminoglycanGAPDH, glyceraldehyde 3-phosphate dehydrogenaseGlcNAc, N-acetylglucosamineHA, hyaluronanHEK, human embryonic kidneyHPLC, high performance liquid chromatographyHS, heparan sulfateHYAL, hyaluronidaseHep, heparinHyaluronanKIAA1199LDS, lithium dodecyl sulfateORF, open reading framePBS, phosphate-buffered salinePBS-T, PBS containing 0.05% Tween 20PCR, polymerase chain reactionsiRNA, short interfering RNA

More Related Videos

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study11:10

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

15.5K
In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration11:16

In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration

Published on: February 10, 2023

1.4K

Related Concept Videos

Glycosaminoglycans01:23

Glycosaminoglycans

Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
6.4K
Cytoskeletal Linker Proteins - Plakins01:09

Cytoskeletal Linker Proteins - Plakins

Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
2.0K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.1K