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mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
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Reversible, lectin-mediated immobilization of plant protoplasts on agarose beads
1Wolfson Institute of Biotechnology, The University, S10 2TN, Sheffield, UK.
Planta
|November 21, 2013
Abstract:
A technique is described for the reversible, lectin-mediated immobilization of plant protoplasts on agarose beads. Cyanogen-bromide-activated agarose beads were coated with protein (gelatine or bovine serum albumin) and lectins were subsequently linked to the protein layer using glutaraldehyde. The technique has possible applications in protoplast fusion-product isolation, cellrecognition studies, and membrane isolation.
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