Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.1K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

3.0K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
3.0K
Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

6.2K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
6.2K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

2.9K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.9K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.9K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leveraging large language models for structured information extraction from pathology reports.

Journal of pathology informatics·2025
Same author

Patient-Reported Paresthesia After Administration of Suzetrigine: A Case Report.

Journal of pain & palliative care pharmacotherapy·2025
Same author

Insect Larvae Meal as a Complementary Functional Ingredient in High Soybean Meal-Based Diets Improve the Health of Rainbow Trout (Oncorhynchus mykiss).

Journal of fish diseases·2025
Same author

Dried Whole Black Soldier Fly Larvae Consumption Supports Gestation, Lactation, and Growth in Cats.

Animals : an open access journal from MDPI·2025
Same author

Pembrolizumab in Combination with Binimetinib in Patients with Unresectable Locally Advanced or Metastatic Triple-Negative Breast Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025
Same author

Effects of black soldier fly larvae on the fecal characteristics, skin and coat health markers, immune function, and oral health measures of healthy adult cats.

Journal of animal science·2025
Same journal

Characterization of Alkane Oxidation Products in a Corona-Discharge Reactor Using Ammonia-Doped Ion Mobility-Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Integration of a Modified Synchrotron Radiation Photoionization Time-of-Flight Mass Spectrometer with a Residual Gas Analyzer for Complementary Detection of Catalytic Products with Different Ionization Energies.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Screen for Tissue-Specific Markers of Drug-Induced Phospholipidosis Using Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Molecular Framework Modification in Mass Spectrometry: Atom Exchange, Insertion, and Deletion.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Interlaboratory Comparison of Orbitrap Analyzers in OrbiSIMS: Noise Parameters, Intensity Scaling, and Detection Limits.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Isotope-Coded Derivatization Enables Accurate LC-MS/MS Quantification of Urinary THC-COOH without Isotope-Labeled Cannabinoid Standards.

Journal of the American Society for Mass Spectrometry·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

6.7K

A new splitting method for both analytical and preparative LC/MS.

Yi Cai1, Daniel Adams, Hao Chen

  • 1Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Ohio University, Athens, OH, 45701, USA.

Journal of the American Society for Mass Spectrometry
|November 21, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a new liquid chromatography/mass spectrometry (LC/MS) splitting method for rapid analysis and sample collection. This technique enables sensitive detection of compounds, even those lacking chromophores, using mass spectrometry.

More Related Videos

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
08:53

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

8.6K
Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

1.6K

Related Experiment Videos

Last Updated: May 5, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

6.7K
Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
08:53

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

8.6K
Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

1.6K

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Mass Spectrometry

Background:

  • Traditional liquid chromatography (LC) methods often require detectors with chromophores, limiting analysis of certain compounds.
  • Achieving fast mass spectrometry (MS) detection and online analyte collection simultaneously presents a technical challenge.

Purpose of the Study:

  • To develop a novel splitting interface for liquid chromatography/mass spectrometry (LC/MS).
  • To enable rapid MS detection of LC-separated analytes with simultaneous online collection.
  • To overcome limitations of traditional detectors by utilizing MS detection for diverse analytes.

Main Methods:

  • A micro-orifice in a PEEK capillary tube was used to split the LC eluent.
  • Desorption electrospray ionization (DESI) was employed for direct ionization of a portion of the eluent.
  • The remaining eluent was directed for online analyte collection.

Main Results:

  • The splitting method achieved negligible time delay (6-10 ms) for MS detection.
  • Extremely small dead volume (as low as 4 nL) resulted in narrow peaks and high sensitivity.
  • Online derivatization using reactive DESI was demonstrated for protein analysis.
  • The method successfully analyzed compounds without chromophores, such as saccharides.
  • The system supported ultra-fast LC separations at high flow rates (4 mL/min).

Conclusions:

  • The novel splitting method offers a versatile approach for LC/MS analysis, enhancing sensitivity and enabling collection.
  • This technique expands the applicability of LC/MS to a broader range of compounds, including those without chromophores.
  • The method is well-suited for high-throughput and ultra-fast LC separations.