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Updated: May 5, 2026
Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
Published on: October 6, 2022
Apoptosis of bladder transitional cell carcinoma T24 cells induced by adenovirus-mediated inducible nitric oxide
Jing Tan1, Qing Zeng, Xian-Zheng Jiang
1Department of Urology, The Third Xiangya Hospital of Central South University, Department of Urology, The Third Xiangya Hospital, Yue-lu District, Changsha 410013, Hunan, China.
Objectives:
To investigate the effects of adenovirus-mediated inducible nitric oxide synthase gene transfection on bladder transitional cell carcinoma T24 cells, and to provide novel insights and approaches to clinical therapies against bladder transitional cell carcinoma.
Methods:
Firstly, construct recombinant adenovirus vector pAd-iNOS of iNOS, followed by transfection of pAd-iNOS into HECK293 packaging cells. Thirdly, harvest recombinant adenovirus rAd-iNOS after amplification and purification procedures. Finally, transfect the recombinant adenovirus rAd-iNOS into human bladder carcinoma T24 cells and examine the effect of rAd-iNOS transfection on apoptosis of T24 and possible mechanism.
Results:
As shown by this study, the recombinant adenovirus rAd-iNOS was constructed successfully. The virus titer was 5.8×10(8) PFU/mL and recombinant was verified by PCR analysis. Transfection of adenovirus rAd-iNOS into T24 cells could induce secretion of high NO concentration, P53 protein expression up-regulation, as well as promotion of T24 cell apoptosis.
Conclusions:
The transfection of human bladder carcinoma T24 cells from recombinant adenovirus rAd-iNOS was confirmed to induce intracellular iNOS over-expression, high production of NO, up-regulation of intracellular P53 expression and promotion of cell apoptosis.
Insights
Adenovirus-mediated transfection of inducible nitric oxide synthase (iNOS) into bladder cancer cells successfully induced apoptosis. This approach offers potential for novel bladder transitional cell carcinoma therapies.
Area of Science:
- Oncology
- Gene Therapy
- Molecular Biology
Background:
- Bladder transitional cell carcinoma (TCC) remains a significant clinical challenge.
- Novel therapeutic strategies are needed to improve treatment outcomes.
Purpose of the Study:
- To investigate the effects of adenovirus-mediated inducible nitric oxide synthase (iNOS) gene transfection on T24 bladder cancer cells.
- To explore potential clinical applications for bladder TCC treatment.
Main Methods:
- Construction of a recombinant adenovirus vector (pAd-iNOS).
- Transfection of the vector into human bladder carcinoma T24 cells.
- Analysis of apoptosis induction and underlying mechanisms.
Main Results:
- Successful construction and verification of the recombinant adenovirus (rAd-iNOS).
- rAd-iNOS transfection induced high nitric oxide (NO) concentration and P53 protein up-regulation in T24 cells.
- Significant promotion of T24 cell apoptosis was observed.
Conclusions:
- Adenovirus-mediated iNOS gene transfection effectively induces iNOS overexpression and NO production in bladder cancer cells.
- This method leads to P53 up-regulation and promotes cancer cell apoptosis.
- The findings suggest a promising gene therapy approach for bladder transitional cell carcinoma.
