Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Eukaryotes01:29

Replication in Eukaryotes

15.5K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
15.5K
Replication in Eukaryotes02:31

Replication in Eukaryotes

157.0K
Overview
157.0K
Replication in Eukaryotes02:31

Replication in Eukaryotes

39.1K
39.1K
Replication in Eukaryotes01:29

Replication in Eukaryotes

10.7K
10.7K
Replication in Prokaryotes01:32

Replication in Prokaryotes

20.6K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
20.6K
Replication in Prokaryotes02:35

Replication in Prokaryotes

86.8K
Overview
86.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mapping Active RNA Polymerases in Proliferating and Quiescent Fission Yeast Cells Using Precision Run-On Sequencing.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Artificial Modulation and Rewiring of Cell Cycle Progression Using Synthetic Circuits in Fission Yeast.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Histone H3 serine-57 is a CHK1 substrate whose phosphorylation affects DNA repair.

Nature communications·2023
Same author

Impact of Chromosomal Context on Origin Selection and the Replication Program.

Genes·2022
Same author

Insights into the Link between the Organization of DNA Replication and the Mutational Landscape.

Genes·2019
Same author

Linking the organization of DNA replication with genome maintenance.

Current genetics·2019

Related Experiment Video

Updated: May 5, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.0K

The programme of DNA replication: beyond genome duplication.

Blanca Gómez-Escoda1, Pei-Yun Jenny Wu

  • 1*Institute of Genetics and Development of Rennes, CNRS UMR 6290, 2 avenue du Professeur Léon Bernard, 35043 Rennes, France.

Biochemical Society Transactions
|November 22, 2013
PubMed
Summary

Accurate DNA replication relies on regulated origin activation. The spatiotemporal organization of replication origins is crucial for cell growth, development, and disease.

More Related Videos

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

7.8K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Related Experiment Videos

Last Updated: May 5, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.0K
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

7.8K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Accurate duplication and transmission of genetic information are vital for cell growth and proliferation.
  • Multi-layered regulation of DNA synthesis ensures genetic fidelity.
  • Selection and activation of replication origins are key steps in DNA synthesis.

Purpose of the Study:

  • To review the crucial roles of spatiotemporal organization of replication origin activation in controlling biological events.
  • To discuss how changes in origin usage during development and disease highlight its importance.

Main Methods:

  • Literature review of studies on DNA replication, origin activation, and chromosomal organization.
  • Analysis of the interplay between replication initiation and cellular functions.
  • Synthesis of current understanding on the spatiotemporal control of replication origins.

Main Results:

  • Replication origin usage is dynamic, changing during development and in pathologies.
  • Spatiotemporal organization of origin activation is integral to cellular function.
  • The regulation of replication initiation sites influences chromosomal processes.

Conclusions:

  • The spatiotemporal control of DNA replication origins is a critical mechanism in biological regulation.
  • Understanding origin dynamics provides insights into development and disease.
  • Targeting replication origin activation may offer therapeutic strategies.