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Updated: May 5, 2026

Floral-Dip Transformation of Flax Linum usitatissimum to Generate Transgenic Progenies with a High Transformation Rate
Published on: December 19, 2014
Plant regeneration from protoplast-derived tissues of Linum usitatissimum L. (Flax)
1Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
Abstract:
Protoplasts were isolated enzymatically from seedling roots, hypocotyls and cotyledons of Linum usitatissimum L. which divided to form callus. Plant regeneration was obtained from protoplast-derived tissues of root and cotyledon, but only rhizogenesis was observed in the case of protoplasts derived from hypocotyls. The ability to isolate, culture, and regenerate plants from root and cotyledon protoplasts of Linum usitatissimum L. is discussed in relation to future attempts to produce somatic hybrids between Linum species.
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