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Further attempts to induce "egg transformation" using irradiated pollen.

Y S Chyi1, J C Sanford, B I Reisch

  • 1Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, 14456, Geneva, NY, USA.

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PubMed
Summary
This summary is machine-generated.

Researchers attempted to replicate egg transformation using irradiated pollen in pea, rapeseed, and apple. No clear evidence of transformation was found, suggesting it

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Reproductive biology

Background:

  • Previous research suggested the possibility of egg transformation using irradiated pollen in *Nicotiana*.
  • The phenomenon, if real, could offer novel methods for genetic modification in plants.

Purpose of the Study:

  • To investigate the occurrence of egg transformation in *Pisum sativum* (pea), *Brassica napus* (rapeseed), and *Malus domestica* (apple) using irradiated pollen.
  • To determine if the egg transformation phenomenon reported in *Nicotiana* is reproducible in other plant species.

Main Methods:

  • Determined optimal irradiation levels (40-100 krad) for pollen to allow fertilization but disrupt chromosome integrity.
  • Employed genetic stocks with multiple Mendelian markers in pea, rapeseed, and apple.
  • Utilized various pollination strategies, including selfing, mixed pollen, and sequential pollinations with irradiated pollen.

Main Results:

  • No clearly transformed seedlings were observed across all tested species and treatments.
  • A total of 6,046 potential transformation events were screened: 2,268 in pea, 3,309 in rapeseed, and 469 in apple.
  • Irradiated pollen effectively prevented normal hybrid formation due to chromosome pulverization, as intended.

Conclusions:

  • Egg transformation, if it occurs outside of *Nicotiana*, is an exceptionally rare event.
  • The frequent occurrence of egg transformation in *Nicotiana* appears to be an isolated phenomenon, not broadly applicable to other species.