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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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CD43 promotes cells transformation by preventing merlin-mediated contact inhibition of growth

Nohemi Camacho-Concha1, Amiel Olivos-Ortiz, Alfredo Nuñez-Rivera

  • 1Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.

Plos One
|November 22, 2013
PubMed

Insights

CD43 promotes cancer by disabling growth control. Inhibiting CD43 in human cancer cells reduced tumor growth, revealing a new role for CD43 in non-hematopoietic tumor progression.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • Tissue size is regulated by cell numbers via contact inhibition of growth, dependent on the NF2/Merlin pathway.
  • Dysregulation of the NF2/Merlin pathway by mutations or oncogenes drives cell transformation and tumor progression.

Purpose of the Study:

  • To investigate the role of CD43 sialomucin in cell transformation and tumor progression.
  • To elucidate the molecular mechanism by which CD43 influences the NF2/Merlin pathway.

Main Methods:

  • Investigated CD43's effect on contact inhibition of growth.
  • Utilized RNA interference to inhibit endogenous CD43 expression in human cancer cells.
  • Assessed tumor growth in vivo.

Main Results:

  • CD43 abrogates contact inhibition of growth by promoting AKT-dependent Merlin phosphorylation and degradation.
  • Inhibition of CD43 expression in lung, cervix, and colon cancer cells impaired in vivo tumor growth.
  • Identified a novel mechanism involving CD43 in non-hematopoietic tumor progression.

Conclusions:

  • CD43 cooperates with oncogenic signals to promote cell transformation by disrupting the NF2/Merlin pathway.
  • Targeting CD43 presents a potential therapeutic strategy for non-hematopoietic tumors.
  • This study highlights a previously unrecognized function of CD43 in cancer biology.

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