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Antibacterial electrospun nanofibers from triclosan/cyclodextrin inclusion complexes
Asli Celebioglu1, Ozgun C O Umu1, Turgay Tekinay2
1Institute of Materials Science & Nanotechnology and UNAM-National Nanotechnology Research Center, Bilkent University, Ankara 06800, Turkey.
Colloids and Surfaces. B, Biointerfaces
|November 23, 2013
Summary
Polymer-free nanofibers were created using cyclodextrin inclusion complexes and the antibacterial agent triclosan. These novel nanofibers demonstrated enhanced antibacterial activity against common bacteria.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Materials Chemistry
Background:
- Cyclodextrin inclusion complexes (CD-IC) offer potential for drug delivery systems.
- Nanofibers (NF) are versatile structures with applications in various fields.
- Developing polymer-free drug-eluting nanofibers is an active area of research.
Purpose of the Study:
- To electrospin polymer-free nanofibers from triclosan/CD-IC without a carrier matrix.
- To characterize the morphology, structure, and complexation efficiency of the resulting nanofibers.
- To evaluate the antibacterial efficacy of the developed nanofibers.
Main Methods:
- Electrospinning of highly concentrated aqueous suspensions of triclosan/CD-IC using hydroxypropyl-beta-cyclodextrin (HPβCD) and hydroxypropyl-gamma-cyclodextrin (HPγCD).
- Morphological analysis via Scanning Electron Microscopy (SEM).
- Structural and complexation confirmation using spectroscopic (¹H-NMR, FTIR) and thermal (XRD, DSC, TGA) techniques.
- Antibacterial testing against Escherichia coli and Staphylococcus aureus.
Main Results:
- Bead-free nanofibers with average diameters of 520 ± 250 nm (HPβCD) and 1,100 ± 660 nm (HPγCD) were successfully produced.
- Triclosan and triclosan/CD-IC formation within the fiber structure were confirmed.
- HPβCD showed higher complexation efficiency with triclosan compared to HPγCD.
- Both HPβCD-IC and HPγCD-IC nanofibers exhibited superior antibacterial activity against both Gram-negative and Gram-positive bacteria compared to pure triclosan.
Conclusions:
- Polymer-free triclosan/CD-IC nanofibers can be effectively fabricated via electrospinning.
- The developed nanofibers possess significant antibacterial properties.
- HPβCD demonstrates superior complexation and potential for enhanced drug delivery applications in nanofiber formulations.
