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Generating suspended cell monolayers for mechanobiological studies.

Andrew R Harris1, Julien Bellis, Nargess Khalilgharibi

  • 1London Centre for Nanotechnology, University College London, London, UK.

Nature Protocols
|November 23, 2013
PubMed
Summary

Researchers developed a new method to test the mechanical properties of cell monolayers. This technique allows for the study of how cell layers respond to stretching, providing insights into their biological functions.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Biophysics

Background:

  • Cell monolayers are crucial in human physiology, involved in mechanical stress.
  • Understanding the mechanical properties of these cell layers is limited.

Purpose of the Study:

  • To develop and validate a protocol for generating freely suspended cell monolayers.
  • To examine the mechanical and biological responses of these monolayers to uniaxial stretch.

Main Methods:

  • Cells are cultured on temporary collagen scaffolds between glass capillaries.
  • Scaffolds are removed with collagenase, leaving monolayers suspended.
  • Micromanipulators apply uniaxial stretch, and force is measured.
  • Optical microscopy images cellular changes during stretching.

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Main Results:

  • A novel protocol for creating and testing suspended cell monolayers was established.
  • The method allows for the measurement of mechanical forces and observation of cellular responses to stretch.
  • The entire process takes 3-4 days.

Conclusions:

  • This protocol provides a new tool for investigating the mechanics of cell monolayers.
  • It enables the study of cellular behavior under mechanical stress, relevant to development and physiology.