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Isolation and characterization of a glucan-type polysaccharide from the red pine mushroom, Lactarius deliciosus
1Centro para la Calidad de los Alimentos, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Campus Universitario Duques de Soria, c/ Jose Tudela s/n, 42004, Soria, Spain.
Abstract:
A novel glucan-type polysaccharide has been isolated from the fruiting bodies of the edible mushroom Lactarius deliciosus. Two successive extractions (cold aqueous extraction at 25°C and hot aqueous extraction at 100°C) were performed, and the same polysaccharide was obtained in both fractions. The purity of the polysaccharide was evaluated by size exclusion chromatography. The SEC chromatogram showed a unique peak with a molecular weight of approximately 150 kDa. Analysis of the chain composition revealed that the polysaccharide was composed of glucose. Methylation analysis and NMR experiments showed that the glucan-type carbohydrate contained a main chain consisting of α-(1→3)-Glcp units with α-(1→4)-linked branches every sixth glucose residue.
