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Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Detection of the intracellular ras p21 oncogene product by flow cytometry
Analytical Biochemistry
|July 1, 1986
Abstract:
Rapid identification of the expression of oncogene products in specific cell types could potentially be useful in the diagnosis and treatment of human malignancy. We have now observed that through the use of lysolecithin permeabilization and fluorescence-activated flow cytometry, cells expressing high levels of the v-Ha-ras oncogene product, p21, can readily be distinguished from the nontransformed parent cells in a rapid and quantitative manner.

