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Chromosomal and isozyme studies of Nicotiana tabacum - Glycine max hybrid cell lines
Y C Chien1, K N Kao, L R Wetter
1Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskatchewan, Canada.
Abstract:
The chromosomal stability of a number of somatic hybrids derived from soybean (Glycine max (L.) Merr.) and Nicotiana tabacum var. 'Xanthi' were investigated. Several of the hybrid cell lines retained more than half the complement of N. tabacum chromosomes after 7 months of culturing. A number of chromosomal abnormalities were observed. The hybrids were positively identified by employing isozyme analysis of several dehydrogenases and aspartate aminotransferase.
