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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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Solubility behavior of cyanophycin depending on lysine content
Lars Wiefel1, Alexander Steinbüchel
1Institut für Molekulare Mikrobiologie und Biotechnologie Westfälische Wilhelms-Universität, Münster, Germany.
Applied and Environmental Microbiology
|November 26, 2013
Summary
Cyanophycin (CGP) solubility is linked to its lysine content. Temperature gradients effectively separate CGP based on lysine levels, aiding purification and specific fraction enrichment in recombinant organisms.
Area of Science:
- Biotechnology
- Polymer Science
- Biochemistry
Background:
- Cyanophycin (CGP) synthesis in recombinant organisms has primarily focused on its insoluble form for biodegradable chemical precursors.
- A soluble form of CGP, notable for high lysine content, has recently gained interest, with lysine hypothesized as the cause of solubility.
Purpose of the Study:
- To investigate the relationship between lysine incorporation and the solubility of cyanophycin (CGP).
- To explore temperature gradients as a method for separating CGP based on its lysine content.
Main Methods:
- Separation of insoluble CGP using a temperature gradient ranging from 90°C to 30°C.
- Analysis of lysine content in CGP fractions obtained at different temperatures.
Main Results:
- Lysine content in CGP directly correlates with the temperature required for solubilization.
- CGP with <3-4 mol% lysine remained insoluble at 90°C, while CGP with 31 mol% lysine dissolved at 30°C.
- CGP with >31 mol% lysine exhibited solubility at room temperature.
Conclusions:
- Lysine incorporation significantly impacts CGP solubility.
- Temperature gradient separation offers a viable method for downstream processing of CGP.
- This technique can enhance purification, and facilitate the enrichment of specific CGP or dipeptide fractions from large-scale fermentations.
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