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Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
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Copper (II) complex-catalyzed oxidation of NADH by hydrogen peroxide
1Department of Biochemistry, State University of New York Downstate Medical Center, 11203, Brooklyn, New York.
Biological Trace Element Research
|November 26, 2013
Summary
Copper ions catalyze NADH oxidation by hydrogen peroxide, with imidazole enhancing this reaction. This suggests a novel reactive oxygen species is formed, crucial for understanding biological oxidations.
Area of Science:
- Biochemistry
- Chemical Kinetics
Background:
- Copper ions (Cu2+) uniquely catalyze NADH oxidation by hydrogen peroxide (H2O2).
- Imidazole and its derivatives significantly influence this catalytic activity.
Purpose of the Study:
- To investigate the mechanism of Cu(2+)-catalyzed NADH peroxidation.
- To identify the reactive oxygen species involved in the oxidation process.
Main Methods:
- Spectrophotometric monitoring of NADH oxidation.
- Analysis of reaction products and intermediates.
- Testing the effects of various inhibitors and radical scavengers.
Main Results:
- Imidazole and specific derivatives activate Cu(2+)-catalyzed NADH oxidation.
- Other imidazole compounds and chelators inhibit the reaction.
- The reaction stoichiometry and lack of effect from scavengers exclude (1)O2 and OH· as intermediates.
Conclusions:
- A copper-hydrogen peroxide intermediate, modulated by ligands, generates a highly reactive oxygen species.
- This mechanism offers insights into biological oxidation processes involving copper and reactive oxygen species.
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