Related Experiment Video
Updated: May 5, 2026

Measuring the Osmotic Water Permeability Coefficient Pf of Spherical Cells: Isolated Plant Protoplasts as an Example
Published on: October 8, 2014
Temperature dependence of intracellular pH in higher plant cells : A (31)P nuclear magnetic resonance study on maize
P Aducci1, R Federico, G Carpinelli
1Gruppo di Chimica Biologica e Strutturistica Chimica, Instituto di Chimica Biologica, Università di Roma, Piazzale Aldo Moro 5, I-00185, Roma, Italy.
Abstract:
The recent introduction of (31)P nuclear magnetic resonance spectroscopy offers a new approach to the problem of obtaining a simultaneous and direct evaluation of both the cytoplasmic and vacuolar pH in higher plant cells (J. K. M. Roberts, P.M. Ray, N. Waderlardetzky and O. Sardetzky, 1980, Nature 283, 870-872; 1981, Planta 152, 74-78). Using this method we have been able to detect a selective pH decrease of about 0.5 units at the level of the cytoplasmic compartment of maize root tips when the temperature was increased from 4 to 28°C. This effect was completely reversible with temperature. No pH variation could be detected at the level of the vacuolar compartment.
Related Concept Videos
Responses to Heat and Cold Stress
Tonicity in Plants
Tonicity in Plants
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Water and Mineral Acquisition

