[The 20210G>A and 19911A>G polymorphisms of prothrombin gene and recurrent miscarriages]

The aim of the study was to evaluate the frequency of 20210G>A and 19911A>G prothrombin gene polymorphisms in a group of women with 2 or more miscarriages in the first trimester of pregnancy

The study involved 150 women with two or more miscarriages in the first trimester of pregnancy (mean age 31.5 +/- 4.1 years). The control group consisted of 180 healthy women (mean age 28.7 +/- 4.0 years). The frequency of genotypes and alleles of the investigated polymorphisms was evaluated by polymerase chain reaction/restriction fragments length polymorphism method (PCR/RFLP).

As to the 20210G>A prothrombin gene polymorphism, overrepresentations of 20210GA genotype have been observed in the group of women with miscarriages (2.7% vs. 1.1% in the control group, WR = 2.44, ns), as well as overrepresentation of 20210A allele (1.3% vs. 0.6% in the control group, WR = 2.42, ns). Considering the 19911A>G polymorphism, a similar frequency of mutated homozygous 19911GG genotype has been noted (22.6 vs. 21.1% in controls, OR= 1.10, ns). Also, frequency of mutated 19911G allele was similar in both investigated groups with miscarriages and controls (48.6% vs. 45.7%, OR = 0.89, ns).

Our findings suggest lack of correlation of 20210G>A and 19911A>G prothrombin gene polymorphisms with the risk of recurrent miscarriages in the first trimester of pregnancy Further studies, concerning other genetic variants conditioning inherited thrombophilia and environmental factors influencing the risk of recurrent abortions, are needed. Therefore, further research with more numerous group of women with recurrent miscarriages and taking into account other polymorphisms of thrombophilia and influence of environmental factors is required.