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S-nitrosothiols, nitric oxide and proinflammatory cytokines in children with inflammatory bowel disease

S A Kolesov1, L V Korkotashvili, A B Yazykova

  • 1Federal State Institution "Nizhny Novgorod Research Institute of Children Gastroenterology", Laboratory Department, Nizhny Novgorod, Russia. sakdom2@gmail.com

Clinical Laboratory
|November 27, 2013
PubMed

Insights

Children with inflammatory bowel disease (IBD) show elevated S-nitrosothiols (RSNO) in blood serum. These findings suggest RSNO plays a role in pediatric IBD development.

Area of Science:

  • Pediatric Gastroenterology
  • Biochemistry
  • Immunology

Background:

  • Inflammatory bowel disease (IBD) encompasses Crohn's disease (CD) and ulcerative colitis (UC).
  • Nitric oxide (NO) metabolism is implicated in IBD pathogenesis.
  • S-nitrosothiols (RSNO) are key NO metabolites with diverse biological functions.

Purpose of the Study:

  • To investigate S-nitrosothiol (RSNO) levels in pediatric IBD patients.
  • To correlate RSNO levels with nitric oxide metabolites (NOx) and proinflammatory cytokines (PC).
  • To explore the role of RSNO in the pathogenesis of pediatric IBD.

Main Methods:

  • Serum samples from 174 children (112 IBD, 62 controls) aged 6-17 years were analyzed.
  • S-nitrosothiols (RSNO) and NOx were quantified using the Griess method.
  • Proinflammatory cytokines (PC) were measured via ELISA; data analyzed using Statistica 6.1.

Main Results:

  • Elevated, though not statistically significant, RSNO levels were observed in children with nonspecific ulcerative colitis (NUC) and Crohn's disease (CD).
  • No correlation was found between RSNO and total nitric oxide (NO) levels.
  • A unidirectional correlation was observed between RSNO and proinflammatory cytokine (PC) levels.

Conclusions:

  • RSNO concentrations in pediatric IBD patients suggest their involvement in disease pathogenesis.
  • Findings indicate an indirect link between aberrant nitric oxide synthase activity and RSNO formation in pediatric IBD.
  • RSNO may serve as a biomarker or therapeutic target in pediatric IBD.
Abstract

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