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Influence of low hemoglobin in children with iron deficiency anemia on PFA-100 testing

Mehmet Akin1

  • 1Denizli State Hospital, Department of Pediatric Hematology, Denizli, Turkey. drmehmetakin@yahoo.com.tr

Clinical Laboratory
|November 27, 2013
PubMed

Insights

Low hemoglobin levels in children with iron deficiency anemia do not impact PFA-100 test results. This screening test remains reliable for diagnosing platelet disorders despite anemia.

Area of Science:

  • Pediatric Hematology
  • Hemostasis and Thrombosis
  • Anemia Research

Background:

  • Iron deficiency anemia (IDA) can affect platelet function.
  • Primary hemostasis alterations in anemic children require evaluation.
  • The PFA-100 instrument is used to assess platelet function.

Purpose of the Study:

  • To evaluate primary hemostasis in children with IDA using the PFA-100.
  • To determine the correlation between low hemoglobin and PFA-100 closure times.

Main Methods:

  • Analyzed 65 children (2-14 years) with IDA.
  • Defined IDA by specific hemoglobin, hematocrit, erythrocyte, MCV, MCH, and ferritin levels.
  • Used PFA-100 with collagen/epinephrine (C/EPI) and collagen/ADP (C/ADP) cartridges, comparing to a control group.

Main Results:

  • No significant correlation was found between hemoglobin values and C/EPI closure times.
  • No significant correlation was observed between hemoglobin values and C/ADP closure times.

Conclusions:

  • Low hemoglobin levels do not influence PFA-100 C/EPI or C/ADP closure times.
  • The PFA-100 test is not affected by low hemoglobin in children with IDA.
  • PFA-100 remains a valuable screening tool for platelet secretion disorders and von Willebrand disease in anemic children.
Abstract

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