Related Experiment Video
Updated: May 5, 2026

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
Chiral separations by non-aqueous capillary electrophoresis in DMSO-based background electrolytes
L Asensi-Bernardi1, A Van Schepdael
1Pharmaceutical Analysis, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, O&N 2, PB-923, Herestraat 49, B-3000 Leuven, Belgium.
Abstract:
Capillary electrophoresis (CE) is a powerful technique for enantioseparations due to its high separation efficiency, high versatility, speed of analysis and low consumption of samples and reagents. Non-aqueous capillary electrophoresis (NACE) appears as a promising technique to perform enantioseparations when the drugs, chiral selectors or samples are non-water soluble. Chiral separations have been performed by NACE mainly using alcoholic solvents as BGEs, with problems of current breakdowns and changes in the BGE composition, due to their high volatility. In this work, the suitability of DMSO as BGE in NACE has been evaluated. Different experimental variables affecting the enantioresolution of three drugs have been evaluated, finally achieving complete enantioresolution of two drugs (verapamil, Rs=1.5 and pindolol, Rs=2.0) and partial resolution of the third one (fenfluramine, Rs=1.2). DMSO has been demonstrated to be a good alternative to methanolic BGEs in NACE.
More Related Videos
07:46Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
07:54Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
Published on: October 27, 2020
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation
Ion-Exchange Chromatography
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...