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Related Experiment Video

Updated: May 5, 2026

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing

Published on: June 9, 2016

Kinome profiling.

Maikel P Peppelenbosch¹

¹Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, L-459, P.O. Box 2040, NL-3000 CA Rotterdam, The Netherlands.

|November 27, 2013

Summary

Kinome arrays offer a powerful, automated method for analyzing active kinases in cells. This species-independent technology provides reliable transcriptome screening and is advancing signal transduction research.

Area of Science:

Genomics
Molecular Biology
Biochemistry

Background:

Genomic array technology enables rapid transcriptome screening.
Kinases are crucial in cellular signal transduction pathways.
Kinome array analysis allows for the study of kinase activity.

Purpose of the Study:

To comprehensively review available kinome array platforms.
To summarize results obtained using kinome array technology.
To discuss the potential and future directions of kinome array analysis.

Main Methods:

Spotting consensus kinase peptides on a solid support.
Incubating spotted peptides with cell lysates and radioactive ATP.
Visualizing radioactive peptides to identify active kinases.

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Main Results:

Kinome array technology meets high expectations for kinase activity analysis.
The technology is particularly powerful due to its species independence.
Various kinome array platforms have been developed and utilized.

Conclusions:

Kinome arrays are a valuable tool for studying active kinases.
The species independence of this technology enhances its utility.
Further improvements and future directions for kinome array technology are identified.