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Optimized Solubilization of Albizia myriophylla Benth. Extract Using I-Optimal Design for Anti-Streptococcus mutans
Chaowalit Monton1,2,3, Thaniya Wunnakup1, Jirapornchai Suksaeree4
1Drug and Herbal Product Research and Development Center, College of Pharmacy, Rangsit University, Lak Hok, Mueang Pathum Thani Pathum Thani, 12000, Thailand, rsu.ac.th.
Scientifica
|July 24, 2026
Summary
This study optimized a solvent system for Albizia myriophylla extract, finding a 95% ethanol, propylene glycol, and water mixture effective against Streptococcus mutans. This formulation supports developing oral sprays for dental caries prevention.
Area of Science:
- Phytochemistry
- Microbiology
- Formulation Science
Background:
- Albizia myriophylla possesses antimicrobial compounds like lupinifolin, effective against Streptococcus mutans, a primary cause of dental caries.
- Optimizing extract solubilization is crucial for developing effective oral health products.
Purpose of the Study:
- To optimize a solvent system for solubilizing Albizia myriophylla extract.
- To determine the optimal formulation for maximizing lupinifolin recovery and maintaining antimicrobial activity.
Main Methods:
- Evaluated solubility of A. myriophylla extract in five solvents: water, 95% ethanol, glycerin, polyethylene glycol (PEG) 400, and propylene glycol (PG).
- Employed an I-optimal design to optimize a mixture of 95% ethanol, PG, and water.
- Assessed lupinifolin recovery and anti-S. mutans activity of the optimized formulation.
Main Results:
- Propylene glycol (PG) showed the highest solubility, followed by 95% ethanol and PEG 400.
- The optimal formulation (0.30:0.63:0.07 mass ratio of 95% ethanol:PG:water) achieved 94.18%–101.01% lupinifolin recovery.
- The optimized extract formulation demonstrated significant anti-S. mutans activity.
Conclusions:
- A stable and effective solvent system for Albizia myriophylla extract was successfully developed.
- The optimized formulation provides a basis for creating oral sprays targeting Streptococcus mutans and preventing dental caries.
