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Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
Published on: June 18, 2020
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MALDI imaging mass spectrometry in glomerulonephritis: feasibility study.
Veronica Mainini1, Fabio Pagni, Franco Ferrario
1Proteomics Unit, Department of Health Sciences, University Milano-Bicocca, Monza, Italy.
Histopathology
|November 28, 2013
Summary
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) analyzes kidney biopsies, revealing molecular changes in both diseased and normal-appearing tissues. This proteomics tool shows promise for diagnosing kidney diseases like membranous glomerulonephritis and minimal change disease.
Area of Science:
- Proteomics
- Nephropathology
- Biomarker Discovery
Background:
- Traditional histology is limited in identifying molecular changes in kidney diseases.
- In-situ proteomics offers a molecular perspective alongside morphological analysis.
Purpose of the Study:
- To evaluate matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) for routine diagnostic kidney biopsies.
- To explore the feasibility of MALDI-IMS in nephropathology for identifying molecular alterations.
Main Methods:
- Application of MALDI-IMS to frozen sections of kidney biopsies.
- Analysis of tissue samples from patients with membranous glomerulonephritis and minimal change disease.
Main Results:
- MALDI-IMS detected molecular changes in both pathologically altered and morphologically normal-appearing kidney tissue.
- The technique demonstrated potential feasibility for routine diagnostic use in nephropathology.
Conclusions:
- MALDI-IMS is applicable to kidney biopsies requiring frozen sections for diagnosis.
- Differentially expressed proteins/peptides identified by MALDI-IMS may serve as powerful classifiers for kidney disease nosology.
