FRET-based dimeric aptamer probe for selective and sensitive Lup an 1 allergen detection

Area of Science:

• Biochemistry
• Analytical Chemistry
• Food Science

Background:

• Lup an 1 (β-conglutin) is a significant anaphylactic food allergen.
• Accurate and rapid detection methods for food allergens are crucial for public health.

Purpose of the Study:

• To develop a sensitive and rapid detection method for Lup an 1 (β-conglutin).
• To utilize fluorescence resonance energy transfer (FRET) for allergen detection.

Main Methods:

• Development of a high-affinity dimeric aptamer against β-conglutin.
• Incorporation of donor/acceptor fluorophores onto the aptamer structure.
• Utilizing FRET signal changes upon β-conglutin binding for detection.

Main Results:

• The FRET-based method demonstrated high sensitivity and specificity for β-conglutin detection.
• A low detection limit of 150 pM was achieved.
• The assay provided results in just 1 minute at room temperature.

Conclusions:

• The developed FRET method is an effective tool for the rapid and sensitive detection of Lup an 1 in foodstuffs.
• This assay offers a significant advancement in food allergen testing and safety.